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Frequently Asked Questions
How do I get my test kits!!?
Email us and tell us which tests you want, where to send them and how you want them sent.
Payment is accepted through paypal at QuarterMoonFarmSALES@starband.net.
*Tests should go out the same day as order placed prior to 3pm. Orders received after 3pm may be postmarked next day.
*Priority Shipping is included in the price and will cover up to 8 tests.
*US Post Office priority shipping is the only shipping method available to preserve your product warranty
***QMF will ship tests overseas! Mexico orders add $9.00 and European countries add $11.00 for priority postage -- this pricing is for a limited time only***
How does it work?
The WeeFoal™ test is a non-invasive, accurate and affordable method for diagnosing pregnancy in your mare from 100 days after mating through to 300 days. 'WeeFoal' analyses a small sample of your mare's urine for the presence of a pregnancy associated estrogenic steroid metabolite. This steroid is made by the foetal-placental unit in increasing amounts as pregnancy progresses. It is excreted into urine where it can be easily measured, and so provides a non-invasive means of diagnosing pregnancy status and monitoring fetal viability. Foal Proof is a serum/plasma blood test.
Is WeeFoal™ for mini horses only?
WeeFoal™ can be used to diagnose and monitor pregnancy status in all breeds of horses. It is particularly appropriate for small horses where palpation per rectum or internal ultrasound scanning is not an option. WeeFoal™ is also a great solution for those mares that are too nervous to safely palpate or draw blood on.
Are the tests accurate?
WeeFoal™ does not suffer the problem of 'false positive' diagnoses, often seen with other biochemical pregnancy tests, where mares that have lost their foal are still diagnosed as pregnant. In the event of fetal loss, production of the estrogenic steroid decreases rapidly to non-pregnant levels, thereby resulting in a 'not pregnant' diagnosis when tested by WeeFoal™.
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Specificity: 98% (probability of a negative result for non-pregnant mares, 389 mares tested).
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Sensitivity: 97.1% (probability of a positive result for mares 120 or more days pregnant, 312 mares tested).
How many days along does she have to be to use the new Urine test?
The Urine test has been used successfully as early as 80 days in Australia however, for best results we strongly suggest at 120 days.
How do I collect the urine to test?
The WeeFoal™ test is best performed on freshly collected urine which may be obtained at any time of the day. Collected urine may be stored for up to 4 hours, preferably refrigerated, prior to testing if necessary. The test can also be performed on collected urine which has been stored frozen for up to a month. For easy ways of obtaining a urine sample from your mare, CLICK HERE.
The test may also be performed on collected urine which has been stored for up to 48 hours at 4 degrees centigrade. It does not have to be in sterile conditions. A wee bit of dirt wont hurt.It is not necessary to collect the urine sample directly into a container as it is being voided. If you see your mare urinating, urine which has been voided onto the ground can still be collected by placing absorbent material (e.g. a paper towel) onto it, and squeezing the urine out into a container.
Horses urinate relatively infrequently, about once every 6 hours. Consequently, unless you know the habits of your mare, it can be tricky catching her urinating so that you can collect a sample for pregnancy testing. Some mares are very obliging, and will urinate predictably after exercise or feeding.
Less co-operative mares can often be encouraged to urinate by putting them in a stall with fresh bedding on the perimeter. Some mares will urinate after they have been 'whistled' at.
The WeeFoal™ test is best performed on freshly collected urine which may be obtained at any time of the day.
What is the WeeFoal™ Test Procedure?
The WeeFoal™ test can be performed on a urine sample collected from 120 days after mating through to expected foaling. It is not recommended to use the test before 120 days have elapsed after mating. Run the test at room temperature and in a well-lighted area. The urine sample and test pack should be at room temperature before performing the test. The urine sample must be diluted as described below.
DO NOT RUN THE TEST WITH UNDILUTED URINE.
When ready to perform the test, open the foil pouch and place the test cassette, 2 plastic pipettes and tube of urine diluent on a flat, clean surface. Take one of the pipettes, squeeze the upper bulb and draw enough of the urine sample into the pipette to fill the stem completely. (0.15 ml will fill the stem, and any overdrawn amount will go into the lower bulb reservoir). Discharge the urine sample from the pipette stem into the tube of urine diluent by gently squeezing the upper bulb (any excess fluid from the draw will remain in the reservoir). Replace the cap on the tube of urine diluent and invert the tube twice to thoroughly mix the urine with the diluent. Take the second pipette, put the stem in the tube of diluted urine, and draw enough of the diluted urine sample into the pipette to fill the stem completely. Discharge the diluted urine sample drop by drop from the pipette stem into the round sample well of the cassette by gently squeezing the upper bulb (any excess fluid from the draw will remain in the reservoir).
Allow the sample to soak into the absorbent pad in the well between drops. Once a few drops have been added to the sample well, the serum will start to flow along the membrane in the cassette window. Continue adding drops of diluted urine until the leading edge of the fluid is ¾ of the way along the membrane, i.e. has reached the ‘C’ mark on the plastic housing.
DO NOT FLOOD THE SAMPLE WELL BY ADDING TOO MUCH SAMPLE, OR ADDING IT TOO QUICKLY.
Wait for 5 to 10 minutes. The test result will generally be obvious within 5 minutes but occasionally it may take up to 10 minutes for the result to clearly form. Each test comes with clear, easy to follow directions and email & phone support is at your fingertips! Email us at QuarterMoonFarm@starband.net or phone 307-245-3698.
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